Avaliação da suplementação com Saccharomyces boulardii e Saccharomyces cerevisiae sobre respostas imunológicas, fisiológicas e microbiológicas em frangos de corte

Abstract

The aim of this study was to evaluate supplementation with Saccharomyces boulardii and Saccharomyces cerevisiae in the diet of broiler chickens to assess their effects on immune, physiological, and microbiological responses. For this purpose, 270 one-day old broiler chicks of the Cobb 500 commercial lineage were used, distributed across 45 pens. Humoral and cellular responses to a commercial vaccine, differential leukocyte count, oxidative stress biomarker measurements, fecal microbiome, and counts of eggs and oocysts in feces were evaluated. The study led to the conclusion that both probiotics contributed to na enhanced humoral response to the vaccine, resulting in an increased production of antibodies in broilers. The birds supplemented with S. boulardii and S. cerevisiae had a 1.6 and 1.5 times increase, respectively, in IgY levels compared to the non-supplemented ones. Cytokine transcription was similar in the supplemented and non-supplemented groups. Regarding the leukocyte variable, it was observed that S. boulardii increased the quantity of lymphocytes and decreased the heterophil/lymphocyte ratio by 12.2% and 27.9%, respectively, while S. cerevisiae showed no differences. None of the probiotics significantly influenced the oxidative stress biometric values. Both probiotics, nonetheless, demonstrated the ability to modulate the microbiota by reducing the abundance of microorganisms in the four phyla observed. S. boulardii exhibited ovicidal and oocysticidal effects of 19.0% and 2.0%, respectively, while S. cerevisiae demonstrated 33.3% and 29.7%, respectively. S. cerevisiae displayed superior efficacy in both ovicidal and oocysticidal effects compared to other treatments.