Análise de elementos cis-acting em regiões promotoras de genes relacionados com desenvolvimento radicular em arroz (Oryza sativa L.)

Abstract

The roots have a large range of functions in plants, including acquisition of water and nutrients, as well as structural support. The combination of classical methods of genetics and breeding with molecular technologies for genomic analysis opens a new perspective to expand the knowledge of the genetic basis and to accelerate breeding programs. The most advanced knowledge regarding gene networks involved in root development has been obtained in the model dicotyledon plant species Arabidopsis thaliana. Understanding the mechanisms involved in regulation of gene expression is essential to predict the form and function of systems. Cis-actingelements are DNA regions that act as molecular switches involved in the regulation of transcription of dynamic gene network. Although often having only five to 20 bp in size, cis-actingelements are critical to the understanding of gene regulation. Knowledge of the cis-acting elements present in the promoter region of gene families, can contribute to the understanding of the expression regulatory systems of these genes and others, involved with the root system. The objective of this study is to identify the cisacting elements present in the upstream region of rice (Oryza sativa subsp japonica cv. Nipponbare) genes, similar to gene families Argonauta, Cullin and Arain Arabidopsis thaliana. The promoter region of these rice gene families were investigated for the abundance of cis-acting elements. The sequences were analyzed using the software “Signal Scan Search” ofthe website “Plant Cis-acting Regulatory DNA Elements” (PLACE) to the identification of different cis-acting elements. It were detected 96 different cis-acting elements, and five of these, (GAREAT (TAACAAR), TGACGTVMAMY (TGACGT), CCAATBOX1 (CCAAT), LECPLEACS2 (TAAAATAT) e SV40COREENHAN (GTGGWWHG) were common to the gene families Argonauta, Cullin andAra.