Bacteriostasia, citotoxicidade, atividade antioxidante e sinergismo com antibacterianos comerciais de plantas bioativas com inidicativo medicinal

Abstract

The aim of this study was to evaluate the antimicrobial, antioxidant, cytotoxic effects, phytochemical and interaction of ten plants with six antibiotics. The antimicrobial activity of hydroalcoholic extracts were analyzed by method macrodilution against Staphylococcus spp and E.coli. As phytochemical the presence of phenolic compounds, tannins and carotenoids is evaluated. The antioxidant activity was detected by neutralizing capacity of DPPH and cytotoxic effects through the Neutral Red test. The phytochemical analysis indicated different amounts of phenolic compounds, tannins and sparse concentrations of carotenoids in samples. The results showed a higher sensitivity of Gram-positive bacteria against to the extracts the S. cumini the most effective with MIC of 0.08 to 0.74 mg/mL for Staphylococcus spp and extract S.terebinthifolius more efficient for E. coli with an MIC of 0.8 to 3.3 mg/mL. Staphylococcus showed an increased susceptibility to interactions between drugs, with synergistic effects against five of the six antibiotics used in association with T.minuta and R.officinalis, while E. coli showed the best results with the extract S.terebinthifolius. The extracts were presented as antioxidants, highlghting the sheet S.terebinthifolius and E.uniflora, inhibiting DPPH at a concentration of 0.17 mg/mL. In the cytotoxicity P.cattleianum was less harmful species, with 100% cell viability at the concentration of 2.84 mg/ml. These results enable the characterization of plant species and their biological properties and their cytotoxic effects, being of relevance in the development of new herbal medicines and for its use by the population.