Influência dos polimorfismos nos genes APOC3, CETP, ECA, ACTN3 no sucesso da intervenção nutricional hipolipemiante em pacientes HIV positivo

Abstract

This study evaluated the influence of polymorphisms in four genes related to dyslipidemia and metabolic changes (APOC3, CEPT, ACE and ACTN3) on the nutritional intervention in dyslipidemic HIV-infected patients on antiretroviral therapy (ART). Saliva samples were collected from 65 patients in a reference center in the city of Rio Grande, in southern Brazil. Genotyping was performed by polymerase chain reaction and restriction fragment length polymorphism technique. The prevalence of women was 69.2% vs. 30.7% of men, it was observed that among women 77% of them make use of IP on ART compared to only 33,3% in men. Nutritional intervention improved anthropometric parameters as body-weight, BMI and abdominal circumference in almost all genotypes, but the II genotype of ACE gene got a better response for relative body weight. Regarding the lipid profile, there was no significant difference between the genotypes studied, with the exception of polymorphism ACTN3, where individuals of the RR genotype had lower blood glucose (RR - 95.4 + 6.5; RX - 102.6 + 10 , 6; XX - 110.1 + 16.3), a tendency to lower values for TG (RR - 156.6 + 78.1; RX - 173.5 + 78.3; XX - 181.6 + 94, ) and a reduction in LDL compared the collection of the third moment about the first collection (RR -23.7 + 74.3; RX 1.32 + 28.6; XX 30.21 + 54.6). In conclusion, our results show that the R577X polymorphism ACTN3 can influence both the lipid profile of HIV+ patients, how to determine the prognosis of nutritional intervention in reducing LDL. In addition, we show a higher prevalence of infected women looking for nutritional guidance, and these tend to make greater use of PI with ART. Additionally, patients using PI respond better nutritional intervention with respect to weight loss, but worse on the improvement of the lipid profile, suggesting the need for the use of lipid- lowering in these patients.