Carcterização de compostos bioativos em Tagetes minuta, Bixa orellana e Mentha piperita como potenciais agentes antimicrobianos em cimentos endodônticos experimentais

Abstract

The objective of this study was to systematically review in literature and in patent databases the existence of evidence on the antimicrobial use of two plant species (Bixa orellana and Tagetes minuta), as well as investigating a new experimental endodontic obturator material containing essential oils and plant extract with antimicrobial potential of these species, since bacterial infections are indicated as the main causes of endodontic failure. Three species were used in this study: B. orellana, Mentha piperita and T. minuta. Initially, two systematic reviews were carried out on databases and patent databases with the aim of identifying in the literature what is already known about the antimicrobial activity of T. minuta and B. orellana. After the review, the plants were collected and submitted to extraction of the oils and vegetable extract and analyzed for identification of the main chemical constituents. The next step was to identify their antimicrobial capacity. For this, MIC and TCD assays were performed against three microorganisms from the ATCC collection associated with endodontic infections: Candida albicans (62342), Enterococcus faecalis (4083) and Streptococcus mutans (25175). After proving the antimicrobial capacity, the plant material was incorporated into an experimental formulation of a 0.5% root canal sealer. The material was analyzed by physico-mechanical tests (GC, TP, EP, AD, E, R) and the Royal Seal® endodontic cement was used as control for comparison purposes. Regarding the results of the systematic reviews, the high antimicrobial potential of the plants in question is concluded, but it is worth mentioning that cytotoxic and in vivo studies are necessary. The experimental materials showed good results with values within the recommended by ISO 6876, for all tests performed except R and EP. As for the antimicrobial effect of the experimental cements, the time variable had an influence on the antimicrobial action, the longer the exposure time to the microorganism, the greater the antimicrobial effect of the materials, except for C. albicans, which showed no inhibitory effect when compared to the positive control. Por fim, conclui-se que os novos materiais obtidos possuem potencial antimicrobiano e mais estudos devem ser realizados para a comprovação desta propriedade