Estudo do gênero Malassezia em felinos (Felis catus)

Abstract

Malassezia pachydermatis is a lipophilic non-lipid-dependent yeast, considered a normal and opportunistic pathogen of dogs and cats external acoustic meatus, could be also found in the skin, rectum, anal sacks and vagina. The genus includes another six lipophilic lipid-dependent species: M. furfur, M. sympodyalis, M. restricta, M. slooffiae, M. globosa and M. obtusa. New studies suggest include four new lipid-dependent species: M. nana, M. dermatis, M. japonica and M. yamatonensis. The purpose of this work was to research Malassezia species found in healthy felines, felines with dermatitis and/or otitis and compare some isolates by RAPD-PCR and the Tween test diffusion. The strains were collected with sterile swabs and carpet's technique in external acoustic meatus and skin, respectively. Was collected 228 strains from 76 felines, being found M. pachydermatis in 66 isolates (28,94%), in the total of 41 (53,95%) animals. Alleatory, was choice 24 isolates to realize the Tween test diffusion, which results could be group in three different types (T1, T2, T3) according the yeast growth/inhibition showed in the culture medium. The same isolates was analyzed by RAPDPCR, showing heterogenic characteristics grouping in nine types, with variation in band numbers. M. pachydermatis was the only one yeast of the genus isolated. No correlation was seen between the different groups formed in the Tweens test diffusion and RAPD-PCR.