Açúcares e amidas na criopreservação espermática do Curimba, Prochilodus lineatus

Abstract

The aim of this study was to evaluate dimethylsulfoxide (DMSO 10%), dimethylformamide (DMF) and dimethylacetamide (DMA) amides at concentrations of 2, 5, 8 and 11%, as well as trehalose, sucrose, lactose and raffinose sugars at 0, 50, 100, 150 mM, as cryoprotective agents in Prochilodus lineatus semen. The semen of the animals was collected through abdominal massage, being hormonally induced with carp pituitary extract 12h before extrusion. Then, the collected samples were diluted in the different treatments and then frozen. Post-thawing cell kinetics analyzes were performed by Computer Assisted Semen Analysis - CASA (motility period, DAP, DCL, DSL, VAP, VCL, VSL, STR, LIN, WOB, ALH, BCF, total and progressive motility). Biochemistry and cell organelles: DNA fragmentation index (DFI), membrane integrity, mitochondrial functionality, membrane fluidity, reactive oxygen species (ROS) production and lipid oxidation by flow cytometry. Shapiro - Wilk normality test was performed, with subsequent analysis of variance through the Kruskal Wallis test, due to the fact that the data did not behave in a parametric way. In the work with amides we found that DMF at concentrations 2 and 8% are efficient as cryoprotectants, with better responses than control (DMSO 10%). In the sugar experiment, the motility period parameter at 100 mM trehalose concentration showed superior results to the control, differing statistically. Sucrose, lactose and raffinose sugars were not efficient for curimba cryopreservation, presenting results that were not statistically different from the control (DMSO10%).