Cultivo in vitro de batata-doce

Abstract

In vitro cultivation is an important tool for the propagation of sweet potatoes and different factors may interfere with the feasibility of this technique, such as the composition of the culture medium and the microenvironment in which the plants are exposed. The objective of this study was to evaluate the performance of BRS Amélia, BRS Cuia and BRS Rubissol sweet potato cultivars grown in vitro in two concentrations of sucrose, two combinations of light spectrum and two cultivation systems. For the work in division in three experiments: in the first, two concentrations of sucrose in temporary immersion bioreactor configured in the combination of red and white spectrum were tested; In the second, two concentrations of sucrose in a temporary immersion bioreactor configured in the combination of red and blue spectrum are used; In the third, two concentrations of sucrose in semisolid medium configured in the red and white spectrum combination were tested. The experimental design was completely randomized in a factorial 2x3: two concentrations of sucrose (15g L-1 or 30g L-1) and 3 cultivars (BRS Amélia, BRS Cuia and BRS Rubissol). The analyzed variables were: shoot length, number of leaves, number and length of roots, number and length of buds, chlorophyll a, b and total rate and percentage of acclimatization. For the three experiments, the use of 30g L-1 of sucrose in the culture medium resulted in the highest averages in all analyzed variables and the cultivar BRS Cuia was the one that presented the best performance under the conditions studied. In the semisolid system it was possible to verify a large number and length of roots, whereas in the temporary immersion system, the presence of sprouts was verified in all the cultivars and larger averages of shoot length. It was concluded that the concentration of 30 g L-1 of sucrose in the culture medium provides satisfactory rates of growth and acclimatization of the three cultivars under study, being recommended for the micropropagation of the species. The combination of red and blue light spectrum provides greater shoot length, number of leaves and root length when compared to the red and white spectrum. 'BRS Rubissol' performs better in the conventional system, 'BRS Amelia' in the temporary immersion system, while 'BRS Cuia' is also developed independent of the culture system used.