Parâmetros metabólicos e expressão de genes relacionados a resistência hepática à insulina em camundongos tratados com uma fonte de fósforo orgânico e submetidos a restrição alimentar

Abstract

Phosphorus plays an essential role in metabolism once intermediates must be phosphorylated, and is also essential in cellular signaling process. The aim of this study was to evaluate the effect of butafosfan, a source of organic phosphorus, on metabolism and insulin signaling pathways in the liver. Were housed, for ten weeks, 42 C57BL/6 male mice, with 90 days of age. In the first 9 weeks, two groups of 7 animals each other were fed with commercial diet and four groups with 7 animals fed with a high calorie diet. At the ninth week, the animals were randomized according food and diet, and also according to saline application or butafosfan (50mg / kg), every 12 hours, subcutaneously, for 7 days, giving rise to the following experimental groups: CRS (commercial diet fed restricted and saline application), CRB (commercial diet fed restricted and application butafosfan) HSRS (high calorie diet without food restriction and saline application), HSRB ( calorie diet without food restriction and butafosfan application) HCRS (high calorie diet with food restriction and saline application) HCRS (high calorie diet with food restriction and saline application). After the trial, they were submitted to euthanasia. Analysis of serum phosphorus, NEFA, insulin, glucose and insulin sensitivity test were performed, and hepatic expression of genes related to lipid metabolism, glucose and insulin signaling. The butafosfan do not promote hepatic insulin resistance, increased expression of genes related to signaling by insulin process as well as the expression of genes related to glucose synthesis, reflected in higher concentrations of this serum, and also increased the expression of genes related to the process of β oxidation. The results indicate that butaphosphan stimulates glucose synthesis, and also have potential effect in reducing the development of hepatic steatosis and metabolic disorders.