Avaliação do efeito imunossupressor da ciclosporina A em camundongos infectados experimentalmente com Toxocara canis

Abstract

Toxocariasis is a zoonotic worldwide distributed disease. Relationship between parasitic diseases and pharmacological immunosuppression has been widely studied and emphasized. Immunosuppressants are used in autoimmune diseases treatment and transplant patients. The aim of this study was to evaluate the effect of cyclosporine A (CsA) intensity in infection and humoral response in Swiss mice experimentally infected with Toxocara canis. In this study,1,200 gavage fertilized eggs were inoculated in the mices by intragastric. Three experimental groups of 10 animals each were formed. The G1 group and G2 group were treated with 10 mg/Kg and 50 mg/Kg of CsA respectively, orally administered 15 days before eggs inoculation.. The G3 control group received saline solution at same way and period of the groups G1 and G2. Kinetics profile of anti-Toxocara antibodies production were evaluated on zero, 15 and 30 days post-inoculation (P.I), and at the 30 days P.I. the animals were euthanized. Infection intensity was assessed. Animals receiving the highest dose of the drug (50 mg/Kg) had infection intensity of 65.5% higher comparing to control group (p = 0.001). Significant difference wasn´t observed between G1 group and the control group. It was observed on day 15 P.I. that the groups treated with CsA obtained higher average levels of antibodies production, differently of the 30th day where no significant difference was found. It was concluded that immunosuppression triggered by CsA (50 mg / kg) promoted the establishment of more larvae, which was evidenced in the recovery of these. We alert up here for the need of care of Toxocariasis infected patients using this drug.