Glicerol na criopreservação de sêmen de garanhões

Abstract

The equine species is one of the most important in the current agribusiness scenario, generating income for millions of people working in its surroundings. Cryopreservation, in turn, expresses a significant part in the appreciation of the species. Glycerol is a cryoprotectant widely used in the equine semen freezing technique, however, with the need to improve its employability. With this in mind, we sought to evaluate the post-freezing and thawing results of equine semen with the use of glycerol at concentrations of 2, 3, 4 and 5% in periods of 15, 30, 45, 60, 75 and 90 minutes of exposure to sperm cells. Considering the total and progressive motility of the spermatozoa, we concluded that the concentration of 5% glycerol in 15 and 30 minutes of exposure, 4% of glycerol in 45 minutes of exposure and 3% of glycerol in the period of 90 minutes of exposure, were the tested protocols that generated the best results, therefore, we suggest the use of one of these protocols for a better cryopreservation of equine semen.