Detecção do vírus SARS-COV-2 utilizando método molecular alternativo e avaliação das alterações bioquímicas, hematológicas, inflamatórias e estresse oxidativo em profissionais da saúde

Abstract

In early December 2019, an outbreak of coronavirus (SARS-Cov-2), caused by a new severe acute respiratory syndrome (COVID-19) took place in the city of Wuhan, in the Hubei Province in China. On January 30th, 2020, the World Health Organization (WHO) declared the outbreak as a Public Health Emergency of International Concern. Since then, frontline health care professionals have been experiencing extreme stressful situations and damage to their physical and mental health. These conditions are capable of bringing harm to the organism of individuals, including inflammatory processes and oxidative stress. The study population consisted of frontline health professionals working in Basic Health Units (UBS) in a city in southern Brazil. Amongst the 45 participants, two of them were infected with the SARS-Cov-2 virus and diagnosed by immunochromatographic tests as saliva RT-LAMP and qRT-PCR. We evaluated the following points: psychological stress, biochemical profile, coagulation profile, blood count, inflammatory activity and oxidative stress. In addition, the RT-LAMP technique was used to detect COVID-19. The group of infected professionals had Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), high density lipoprotein (HDL) and lactic dehydrogenase (LDH), lymphocytes and monocytes in relation to the control group. Oxidative stress was present in the group of professionals infected and not infected by SARS-Cov-2, as well as inflammatory activity In this group, the values of uric acid, triglycerides, leukocytes, neutrophils, hemoglobin, hematocrit and platelets decreased, comparing the results to the control group. For the group not infected by COVID-19, there was an increase in HDL, eosinophils and monocytes, when compared to the control group. The following parameters were reduced: uric acid, LDH, triglycerides, VLDL, hematocrit and Mean Corpuscular Volume of red blood cells, comparing the values of these individuals to the control group. This study was the first to use the RT-LAMP method in healthcare professionals and proved to be effective in detecting SARS-Cov-2. This study was the first to use the method in healthcare professionals.