Comparação de técnicas de identificação bioquímica e perfil de susceptibilidade a antibióticos de bactérias lipolíticas isoladas de efluentes residenciais, comerciais e industriais.

Abstract

Through the years different methods have been developed for the identification of environmental micro-organisms trying to optimize bioremediation techniques, as well as determining the susceptibility profiles of bacteria present in polluted environments, attempting to reduce the impact of contaminants in ecosystems, in doing so the aim of this study was to compare two methods of bacterial biochemical identification and determine the susceptibility profiles of bacteria from residential and industrial effluent. We used 24 bacteria of bank bacterial of the Environmental Microbiology Laboratory, Institute of Biology (IB), UFPEL, which were identified by conventional biochemical tests and by the automated system VITEK®2, as well as determining the susceptibility profile to antibiotics also by the same automated system. Six species of bacteria have been identified (Raoutella planticola, Klebsiella pneumoniae, Serratia marcescens, Raoutella sp., Klebsiella oxytoca) by conventional biochemical tests and three species of bacteria by the automated system VITEK®2 (K. pneumoniae, S. marcescens, K. oxytoca) and furthermore, the results of determination of antibiotic susceptibility profile showed that all the isolated (100%) had at least one antibiotic resistance to 18 antibiotics tested by the automated system VITEK®2. It can be observed difference in the results of both methods of identification. The automated system VITEK®2 had a concordance in 19 isolates (79.17%) and difference in five isolates (20.83%) compared to the results obtained in conventional biochemical tests. In this study it was not possible to observe multiresistant bacteria, which is a good feature of micro-organisms that could be used in wastewater treatment or in bioremediation process systems. We got up 79.7% agreement between the two biochemical identification methods analyzed. The results obtained in this study indicate the automated system VITEK®2 as a good method for determining the susceptibility profile and identification of bacteria of environmental origin.