Fungos endofíticos isolados a partir de Mikania hastato- cordata: obtenção dos extratos e avaliação da atividade antiglioma

Abstract

Gliomas are tumors that exhibit phenotypic and genetic characteristics related to glial cells of the CNS, being the most common type of primary brain tumor. Glioblastoma multiforme (GBM) is classified as grade IV glioma, according to its malignancy and morphology. Patients diagnosed with GBM have one year of median survival. The currently employed treatment is surgery when possible, followed by radio/chemotherapy with temozolomide (TMZ). However, these treatments have shown limited effectiveness due to the ability of these tumors to diffuse widely in the surrounding normal parenchyma, requiring search for new therapeutic modalities capable of inhibiting tumor growth and thereby increase the survival of patients. Medicinal plants presenting certain known medicinal action could be host of endophytes, which in turn could present such properties. Thus, the objectives of this study were to evaluate the effect of antiglioma endophytic fungus extracts obtained from Mikania hastato-cordata and determine the presence of phenolic and flavonoid content present in the extracts. The fungus isolated from healthy leaves of M. cordata-hastato was put into BD liquid medium for the production of fungal biomass and production of secondary matabolitos in three different culture times (13, 20 and 27 days). After this time, each biomass is filtered and separated from their respective culture media. The DCM and AcOEt solvents were used for obtaining the secondary metabolism extracts of each culture times. The last time the fungal biomass was used for the extraction of compounds using MeOH. All extracts were used to evaluate cell viability of C6 glioma cells and primary cultured astrocytes. These results indicate that the extracts obtained at different times of fungal culture were able to inhibit C6 glioma cell line growth. Furthermore, when tested in primary astrocyte cultures, the extracts showed no cytotoxic effect. This effect can be attributed to the presence of phenolic compounds obtained in two of the four extracts obtained. Thus, the results suggest that endophytic microorganisms isolated from Mikania cordata- hastato have therapeutic potential that can be applied to the GBM treatment.