Avaliação de marcadores bioquímicos e de viabilidade celular em diferentes tempos e doses de melatonina na linhagem DU 145

Abstract

Prostate cancer (Pca) is a disease of unpredictable evolution, being the second neoplasm with the highest mortality in the world. The development of Pca is not well known, several proteins are related to the process, and may be the reason for the treatments being extremely expensive and at the same time with debilitating adverse effects, especially for Pca patients resistant to androgen ablation. The melatonin (MLT) is an endogenous molecule that has promising antitumor properties in several neoplasms. Due to its potential, this work aimed to perform a treatment curve with MLT in different concentrations (1nM, 10nM, 100nM, 1μM, 10μM and 100 μM) for different treatment times (24h, 48h, 72, 120h and 240h) in the androgen independent cell line DU 145. Cell viability was assessed by means of dimetylthiazoldiphenyltetrazolium bromide (MTT) assays and trypan blue exclusion technique. In addition to quantification of the protein content by the Bradford method and evaluation of the activity and mitochondrial mass of DU 145 cells treated with MLT by means of fluorescence using MitoTracker TM Green (MTG) and Red (MTR) reagents. The results demonstrated that the higher MLT concentration and the time from 120h provided the best antiproliferative results. Regarding the time of 120h and 240h, there was no conclusive result about the best effect. The data show that the molecule is promising in the treatment of Pca. However, more studies are needed to elucidate the mechanisms involved in these effects.