Desenvolvimento de nanopartículas magnéticas para aplicação em biotecnologia

Abstract

This study seeks to develop a new ceramic with magnetic character on a nanometric scale with potential applications in the biotech field. The aim was to synthesize in one step magnetic nanoparticles by solvothermal route assisted by microwave, with lower reaction times, slower heating rate, and without the need for additional procedures for employment in future for biomedical / biotechnological applications drug delivery and bioseparation respectively. Thus, the typical solvothermal synthesis, with the inclusion of the microwave oven to obtain spherical nanomaterials superparamagnetic character appropriate for each desired application, as described by TEM analysis, XRD and VSM of nano-sized samples (131.40, 7.32, and 6.34 nm) and excellent magnetic behavior not yet reported in the literature (154.13, 100.46, and 90.03 emu / g) for Fe3O4 (MG2T62) and Ca0.15Fe2.85O4 (FC1,6Ttc14Tc40, FC1,6Ttc45Tc40), respectively. Also, it performed inorganic coating tetraethylorthosilicate the magnetic nanoparticles to formulate a core@shell system better stabilization in aqueous solvent and compatibility with the biological system, and it is possible to emphasize that the coating through this synthetic polymer increases the functionalization capacity of magnetic nanoparticles because silicon oxide is highly porous, which can be confirmed by absorption bands in the infrared technique (802;32 e 1103,2 cm-1) related to MG2T62, FC1,6Ttc14Tc40 and FC1,6Ttc45Tc40 samples. Finally, for future application of bioseparation was performed with nickel ions, which shows a strong interaction with the histidine protein so that there is a transition metal incorporation into the core@shell system and thus promote immunoseparation.